| Registro completo |
| Provedor de dados: |
Agronomy
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| País: |
Brazil
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| Título: |
Characterization of race 65 of Colletotrichum lindemuthianum by sequencing ITS regions
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| Autores: |
Coelho, Marcela
Gonçalves-Vidigal, Maria Celeste
Sousa, Lorenna Lopes de
Nunes, Maria Paula Barion Alves
Azevedo, Rafhael Felipin
Galván, Marta Zulema
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| Data: |
2016-09-02
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| Ano: |
2016
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| Palavras-chave: |
5.01.00.00-9
5.01.03.00-8 anthracnose
Genetic variability
Pathogens
Dissimilarity Melhoramento Vegetal
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| Resumo: |
The present work aimed characterize isolates of C. lindemuthianum race 65 from different regions in Brazil by ITS sequencing. A total of 17 isolates of race 65, collected in the states of Mato Grosso, Minas Gerais, Paraná, Santa Catarina and São Paulo, were studied. Analysis of the sequences of isolates 8, 9, 12, 14 and 15 revealed the presence of two single nucleotide polymorphisms (SNPs) in the ITS1 region at the same positions. These isolates, when analyzed together with the sequence of isolate 17, revealed a SNP in the ITS2 region. The highest genetic dissimilarity, observed between isolates 11 and 3 and between isolates 11 and 10, was 0.772. In turn, isolates 7 and 2 were the most similar, with a value of 0.002 for genetic distance. The phylogenetic tree obtained based on the sequences of the ITS1 and ITS2 regions revealed the formation of two groups, one with a subgroup. The results reveal high molecular variability among isolates of race 65 of C. lindemuthianum.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30586
10.4025/actasciagron.v38i4.30586
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| Editor: |
Universidade Estadual de Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30586/pdf
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Agronomy; v. 38, n. 4 (2016); 429-438
Acta Scientiarum. Agronomy; v. 38, n. 4 (2016); 429-438
1807-8621
1679-9275
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| Direitos: |
Direitos autorais 2016 Acta Scientiarum. Agronomy
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